Human Stem Cell Manual: A Laboratory Guide > 생명과학

Table of Contents
Foreword James Battey ix
Foreword Jeannie Fontana xi
Preface Jeanne Loring xiii
Preface Robin L. Wesselschmidt xv
Preface Philip H. Schwartz xvii
List of Contributors xix
Basic Methods in Stem Cell Culture 1
Human Embryonic Stem Cell Culture Rodolfo Gonzalez Robin L. Wesselschmidt Philip H. Schwartz Jeanne E Loring 3
Human Feeder Cells, Feeder-free, and Defined Culture Systems Robin L. Wesselschmidt Jeanne F. Loring 18
Mouse Embryonic Fibroblast Feeder Cells Chris Stubban Robin L. Wesselschmidt 34
Cryopreservation of Human Embryonic Stem Cells Chris Stubban Robin L. Wesselschmidt Igor Katkov Jeanne F. Loring 47
Characterization of Stem Cells 57
Classical Cytogenetics: Karyotyping Robin L. Wesselschmidt Jeanne E. Loring 59
Spectral Karyotyping and Fluorescent in situ Hybridization Suzanne Peterson Stevens Rehen Willem Westra Yun Yung Jerold Chun 71
Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis Kevin Gunderson David L. Barker Marina Bibikova Jian-Bing Fan 85
Flow Cytometric Analysis of Human Embryonic Stem Cells Andrew L. Laslett Andrew Fryga Martin F. Peru 96
Immunocytochemical Analysis of Stem Cells Lisa A. Flanagan Boback Ziaeian Theo Palmer Philip H. Schwartz 108
Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction Shengwen Li Ivan Kirov Jr. Henry J. Klassen Philip H. Schwartz 127
Gene Expression Profiling of Stem Cells by Microarray Timothy McDaniel Shawn Baker Roy Williams Franz-Josef Mueller David Barker 149
Generation of Human Embryonic Stem Cell-derived Teratomas Robin L. Wesselschmidt 162
Characterization of Human Embryonic Stem Cell-derived Teratomas Ivan Damjanov Lars Ahrlund-Richter 171
Differentiation of Human Embryonic Cells 183
Embryoid Bodies and Neuroepithelial Development Matthew T. Pankratz Su-Chun Zhang 185
Motor Neuron and Dopamine Neuron Differentiation Xue-Jun Li Dali Yang Su-Chun Zhang 199
Oligodendrocyte Differentiation from Human Embryonic Stem Cells Maya N. Hatch Gabriel I. Nistor Hans Keirstead 210
Cardiac Development of Human Embryonic Stem Cells Maria Barcova Victor M. Campa Mark Mercola 227
Hematopoiesis from Human Embryonic Stem Cells Kausalia Vijayaragavan Veronica Ramos-Meijia Mick Bhatia 238
Genetic Manipulation of Stem Cells 253
Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors Ruchi Baipai Alexey Terskikh 255
Genetic Manipulation of Embryonic Stem Cells Tobias Raabe Robin L. Wesselschmidt 267
Advanced Methods 289
Derivation of Embryonic Stem Cells from Human Blastocysts Prithi Rajan David Smotrich Ric Ross Louise Laurent Jeanne F. Loring 291
Neural Progenitor Cell Culture Hubert Nethercott Heather Maxwell Philip H. Schwartz 309
Stem Cell Transplantation in the Brain Jean-Pyo Lee Nils Ole Schmidt Paul Christian Baier Franz-Josef Mueller Evan Y. Snyder 332
In Vitro Eertilization Antoine La David Diaz 351
Functional Characterization of Neurons Scott McKerchner Maria Talantova Stuart Lipton 373
Setting up a Laboratory 387
Setting up a Facility for Human Embryonic Stem Cell Research Ian Lyons David Tan Philip H. Schwartz Mahendra Rao 389
Stem Cells and Society 415
Intellectual Property: Owning the Stem Cell Cathryn Campbell Jeanne F. Loring 417
Ethical Concerns for Stem Cell Research Philip H. Schwartz Michael W. Kalichman 426
Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees Michael W. Kalichman Philip H. Schwartz 437
Abbreviations 449
Index 453