Introduction to Plant Biotechnology 3/Ed > 생명과학

Part I: Plant Tissue Culture

• Introduction
• Laboratory Organization
• Nutrition Medium
• Sterilization Techniques
• Types of Culture
• Micropropagation
• Cell Suspension and Secondary Metabolites
• In Vitro Production of Haploids
• Protoplast isolation and fusion
• Somaclonal Variation
• Germplasm Storage and Cryopreservation

Part II: Genetic Material and its Organization

• Genetic Material
• Organization of DNA and Gene Expression

Part III: Recombinant DNA Technology

• Basic Techniques
• Gene Cloning: Cutting and Joining DNA Molecules
• Gene Cloning: Vectors
• Gene Cloning: cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences
• Polymerase Chain Reaction
• In Vitro Mutagenesis
• Transposon Genetic Elements and Gene Tagging
• Gene Isolation
• Molecular Markers and Marker-Assisted Selection
• Gene Transfer Methods
• Chloroplast and Mitochondrion DNA Transformation
• Transgenics in Crop Improvement
• Impact of Recombinant DNA Technology
• Biosafety Concerns and Regulatory Framework
• Genomics
• Bioinformatics
• Intellectual Property Rights