도서 정보
도서 상세설명
Project Introduction
PROJECT I. GENOMIC DNA ISOLATION AND ANALYSIS
Module ㅣ.1 Mouse Genomic DNA Isolation and Analysis
Module ㅣ.2 Southern Blot Experiment to Detect Gene-Specific Sequences in Genomic DNA
Module l .3 PCR Amplify a Specific Gene from Mouse Genomic DNA and Verify Identity by Southern Blot Analysis
PROJECT II. GENOMIC CLONING, DNA SEQUENCING, AND BIOINFOMATICS
Module ll.1 Prepare Mouse Genomic DNA for Cloning; Production and Titering of a Lambda Genomic Library
Module ll.2 Screen a Lambda Phage Libraty to Detect Clones of a Specific Gene
Module ll.3 PCR Subclone into a plasmid Vector, Propagate in Bacterial Cells, and Prepare Purified Recombinant Plasmid
Module ll.4 Dideoxy Thermal Cycle DNA Sequencing of a cloned Gene
Module ll.5 Bioinformatic Analysis of the DNA Sequence Data
PROJECT III. GENE EXPRESSION ANALYSIS: RNA ISOLATION, NORTHERN BLOT,
AND REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION
Module lll.1 lsolation and Analysis of Total RNA from Mouse Liver
Module lll.2 Northern blot experiment to Detect a Specific Gene Transcript
Module lll.3 Reverse Transcriptase-Polymerase Chain reaction(RT-PCR) Amplification of a Specific Gene Transcript
PROJECT IV. cDNA CLONING AND cDNA LIBRARY ANALYSIS
Module lV.1 Construction of a cDNA Library and cDNA Library Analysis
Module IV.2 Characterization and Screening of a cDNA Library Using the Polymerase Chain Reaction